An Fe3+-activated acid phosphatase in Saccharomyces rouxii.
نویسندگان
چکیده
منابع مشابه
Heterothallism in Saccharomyces rouxii.
Over the years a large number of osmophilic yeasts have been isolated from honey, sirups, and highly salted fermented foods of the Orient. AMany have been considered as new species by the persons who isolated them. Strains vary from one another by minute differences in physiology and morphology. The osinophilic yeasts were formerly classified in the subgenus Zygosaccharomyces, a subdivision of ...
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The localization of acid phosphatase (EC 3.1.3.2) in secreting protoplasts prepared from Saccharomyces cerevisiae is reported for the first time. Using a Gomori technique we were able to show acid phosphatase at those organelles in the protoplasts which are generally involved in the processes of biosynthesis and secretion of glycoproteins in eukaryotic cells.
متن کاملRegulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae.
We examined the genetic system responsible for transcriptional regulation of repressible acid phosphatase (APase; orthophosphoric-monoester phosphohydrolase [acid optimum, EC 3.1.3.2]) in Saccharomyces cerevisiae at the molecular level by analysis of previously isolated and genetically well-defined regulatory gene mutants known to affect APase expression. These mutants identify numerous positiv...
متن کاملIsolation and characterization of protoplasts from Saccharomyces rouxii.
Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. mellis (another osmotolerant species) and contrasts with the 0.3 to 0.8 M KCl ...
متن کاملPhysicochemical and kinetic properties of acid phosphatase from Saccharomyces cerevisiae.
Acid phosphatase from yeast Saccharomyces cerevisiae was purified, and its physicochemical and kinetic properties were investigated. The sedimentation coefficient has been determined to be s0(20,w) = 13.6 S. The diffusion constant has been found to be 3.9 X 10(-7) cm2s-1, and the calculated partial specific volume was v = 0.663 cm3/g. From these data, a molecular weight of 252,000 was calculate...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)30100-x